J. Chromatogr. A. 1588, 137-149 (2019). HPTLC of sugars (1), amino acids (2), gluconic acid (3) and glycerol (4) in 20 wine samples on silica gel with i-propanol - n-butanol - boric acid solution (200 mg/10 mL) - acetic acid 14:6:3:1 for (1), 2-butanol - ammonia solution (25 %) - pyridine - water 19:5:17:13 for (2), methanol - water 7:3 for (3) and acetonitrile - boric acid solution (200 mg/10 mL) 4:1 for (4). Detection of (3) by heating at 190 °C for 20 min, followed by densitometric evaluation at 366 nm. Further detection by dipping into: 1) diphenylamine-aniline-phosphoric acid reagent, followed by heating at 120 °C for 10 min; 2) vanillin-sulfuric acid reagent, followed by heating at 135 °C for 20 min; 3) ninhydrin reagent, followed by heating at 110 °C for 5 min; 4) bromophenol blue, followed by heating at 110 °C for 10 min. Derivatized plates were documented in white light and under UV light at 366 nm. Quantification of (4) was performed using a deuterium/tungsten lamp at 380 nm. Micro planar chromatography was performed using a device, where the HPTLC foil was covered by a thick glass plate with a hole in the center, through which the mobile phase was supplied. Further analysis by mass spectrometry. 

J. Liq. Chromatogr. Relat. Technol. 43, 361-366 (2020). HPTLC of the dried root and rhizome of Rhodiola rosea on silica gel with ethyl acetate - methanol - water 77:13:10. Detection by spraying with 1) a solution of p-anisaldehyde (0.5 mL in 85 mL methanol, 10 mL acetic acid and 5 mL sulfuric acid), followed by heating at 105 ºC for 5-7 min, 2) a solution of 2-isopropyl-5-methylphenol (0.5 g in 95 mL ethanol and 5 mL sulfuric acid), followed by heating at 120 ºC and 3) NP solution (1 g diphenylboryloxyethylamine in 100 mL methanol) and PEG solution (5 g PEG-4000 in 100 mL ethanol). Detection under UV 254 and 366 nm. Effect directed detection was performed using 1) DPPH* radical reagent assay: spraying with 0.2 % 2,2-diphenyl-1-picrylhydrazyl solution in methanol, 2) AChE assay: spraying with the enzyme solution (20 units of AChE and 150 mg BSA in 150 mL 0.05 M TRIS buffer, pH 7.8), follwed by incubation at 37 ºC for 20 min and spraying with 50 mg Fast Blue B salt diluted in 100 mL of water and 3) Bacillus subtilis bioassay: dipping into bacterial suspension for 8 s, followed by incubation at 37 ºC for 17 h and spraying with 0.2 % MTT aqueous solution. The bioautographic tests showed presence of both antioxidants (DPPH assay) and antibacterials (Bacillus subtilis assay) in the methanolic plant extract, however no acetylcholinesterase inhibitors were found. As marker compound, rosavin was detected.

J. Planar Chromatogr. 27, 174-180 (2014). HPTLC of betulinic acid (1), 24beta-ethylcholesta-5,22E,25-triene-3beta-ol (2) and lupeol (3) in Clerodendrum phlomidis on silica gel with chloroform - methanol 49:1. Detection by dipping into vanillin - sulfuric acid reagent (vanillin - ethanol - sulfuric acid 1:95:5, w/V/V), followed by heating at 110 ºC for 3 min. Quantitative determination by absorbance measurement at 600 nm. The hRF values for (1) to (3) were 30, 52 and 70. Linearity was in the range of 300-1500 ng/mL. The intermediate/interday/intra-day precisions were below 2 % (n=5). The LOD and LOQ were 22 and 74 ng/zone for (1), 20 and 66 ng/zone for (2) and 30 and 100 ng/zone for (3), respectively. Average recovery was 99.0 % for (1), 98.5 % for (2) and 98.2 % for (3).

J. Chromatogr. 369, 435-439 (1986). TLC examination of phorbol on silica with 10 different solvent systems, and on long-chain-hydrocarbon-bonded silica with 4 different combinations of methanol - acetonitrile -water. Detection under UV at 254 nm and by spraying with vanillin - sulfuric acid -ethanol 3:0.5:100 and heating at120 °C.

Planta Medica 82(3), 238-243 (2016). Pure 6-gingerol was submitted to sulfation either with purified human sulfatases (SULT1A1, SULT1A2, SULT1A3, SULT1B1, SULT1C2, SULT1C3, SULT1C4, SULT1E1, SULT2A1, SULT2B1a, SULT2B1b, SULT4A1, and SULT6B1) or with cytosol of human organs (lung, liver, small intestine, kidney), in presence of [35S]-marked 3’-phosphoadenosine 5’-phosphosulfate, and, after centrifugation (13000 rpm, 3 min), the supernatant was analyzed by TLC on silica gel with acetic acid – n-butanol 2:1. The plate was dried with a hair-drier. Detection by autoradiography on an X‑ray film; the radioactive band corresponding to the [35S]-sulfated 6-gingerol (hRf 76) was cut out and eluted with 0.5 mL water into a glass vial for liquid scintillation counting. The strongest 6-gingerol-sulfating activity was exhibited, among the enzymes tested, by SULT1A1 (followed by SULT1C4, SULT1A3, SULT1E1, SULT1A2 and SULT1B1, the other sulfatases being inactive), and, among the organ cytosols, by the small intestine (followed by liver, lung and kidney). The same procedure was applied to detect, after spin filtration, the same [35S]-sulfated 6-gingerol produced from 6-glycerol and [35S]-sulfate in presence of plate-cultured human cells HepG2 (hepatoma) and Caco-2 (colon adenocarcinoma); the amount of the produced sulfated form clearly depended on the gingerol concentration.

Anal. Chim. Acta 192, 309-313 (1987). Description of 3 fluorescent derivatization reagents for compounds having hydroxyl and/or amino groups, which were stable at room temperature and condense stechiometrically with alcohols, amines and amino acids in the presence of alkali to give strongly fluorescent derivatives. Pre-chromatographic derivatization followed by TLC and HPLC.

Planta Medica 82 (16), 1438-1445 (2016). TLC on silica gel with chloroform – methanol 19:1, followed by detection under UV 254 nm and spraying with 10 % sulfuric acid with heating. TLC was used for 1) the ethyl acetate fraction of a hydromethanolic extract of Eryngium triquetrum aerial parts, and 2) for the monitoring of its fractionation on a cyclodextrin column, and of the fractionation of a selected fraction on silica gel column. The (chloroformic) first subfraction of the last step was found to contain only the polyyne alcohol falcarinol (panaxynol, carotatoxin) at hRF 90.

J. Chromatogr. 408, 449-452 (1987). Two-dimensional TLC on polyamide with 1) 5% acetic acid in water and 2) benzene - heptane - acetic acid 7:2:1. Detection by UV.