J Chromatogr A, 1603, 355–360 (2019). Samples were ethyl acetate root macerates of fully flowered Tanacetum vulgare (Asteraceae). HPTLC on silica gel (classical irregular particles vs. Lichrosphere with spherical particles) previously washed with methanol, dried for 5 min at room temperature, perimeter-sealed with a polymer coat, and heated for 30 min at 100 °C. Separation with toluene or with toluene – n-hexane 7:3, in classical capillary flow or in OPLC (overpressured layer chromatography). For OPLC, off-line infusion was used (closed mobile phase (MP) outlet, automatically stopping development); external pressure 50 bar, rapid MP flush 175 and 350 µL, MP flow rate 250 and 500 µL/min, 1830 and 3475 µL MP, development time 446 s and 424 s. Derivatization by immersion into vanillin – sulfuric acid reagent, followed by 5 min heating at 110 °C; or into PABA reagent (500 mg p-aminobenzoic acid, 18 mL glacial acetic acid diluted, 20 mL water, 1 mL o-phosphoric acid, 60 mL acetone), followed by 5 min heating at 140 °C. Effect-directed analysis using automated immersion: A) for free radical (DPPH•) scavengers; B) for activity against Gram-negative bacteria using Aliivibrio fischeri bioluminescence assay; C) for activity against Gram-positive bacteria with Bacillus subtilis bioassay. Four active polyynes were identified as hexadiynylidene-epoxy-dioxaspiro-decane (1), pontica epoxyde (nonene-triynyl-vinyl-oxirane) (2), tetradeca-triine-en-one (3) and trans-(hexadiynylidene)-dioxaspiro-decene (4), by hyphenating OPLC to quadrupole-orbitrap HRMS without eluent, using a DART interface (Direct Analysis in Real-Time, needle voltage 4kV, grid voltage 50 V, helium as gas, temperature 500 °C, full scan in positive ionization mode in m/z range 100-750). Polyynes (3) and (4) were coeluting in HPTLC but not in OPLC, demonstrating that (4) is not produced by oxidation during the DART-MS procedure. Separation with OPLC compared to HPTLC was performed in a shorter time and with better resolution at the same time. Layers with spherical particles gave higher resolution; zone distortions occurring in OPLC due to dissolved air in MP were prevented by previous MP sonication.

Chem. Asian J. 15(19), 3044-3049 (2020). The studied rotaxane combines a dibenzocrown of 8 ethers (DB24C8) with an axle chain (Ax) containing two amines, one of them in an aniline group, allowing stability of the rotaxane even when the other one is unprotonated. TLC on silica gel in 4 steps, with detection under UV light or after derivatization with phosphomolybdic acid in ethanol. (1) Before the synthesis of the rotaxane, unprotonated Ax was isolated by preparative TLC of the protonated Ax obtained by addition of HCl or toluenesulfonic acid (TsOH); the mobile phases were chloroform – methanol 10:1 and toluene – tetrahydrofurane 3:2, respectively. The isolated molecules were confirmed as totally unprotonated Ax by NMR, suggesting a complete loss of HCl and TsOH on the silica gel layer. (2) After synthesis, unprotonated rotaxane, pure vs. monoprotonated by the addition of 10 different acids (and purified by column chromatography CC), was applied on TLC plates and developed with dichloromethane – acetone – water 3:16:1; the hRF values were very different, depending on the counter-anions from the used acids. (3) The same behavior (except with sulfuric acid) was observed under the same conditions when CC was omitted (unprotonated rotaxane samples were mixed with each of the acids, or with two acids at the same time for acid-competitive TLC analysis). (4) When unprotonated rotaxane was applied under the same conditions as in step (3) with the sodium salts instead of the acids, the behavior was similar (except for the shapes of the spots, due to the salts in excess). The rotaxane can thus be used for the TLC separation and detection of sodium salts, by forming salts of protonated rotaxane with the anion afforded by these sodium salts. The rotaxane protonation seems to be promoted by the methanol of the spotting mixture; indeed, when step (3) was performed with the mobile phase chloroform – methanol 10:1, a second zone appeared because methanol formed a salt with the rotaxane (identified by NMR).

J. Planar Chromatogr. 5, 205-206 (1992). TLC of decanal on silica with benzene. Visualization by exposure to iodine vapor or under UV 366 nm.

J. Planar Chromatogr. 8, 80-81 (1995). TLC of fresh petrol, kerosene and petrol - kerosene mixtures and the suspect petrol sample, with heptane. Detection under UV and after spraying with chromogenic spray reagents. Observation under VIS. The method is quick, simple, inexpensive, sensitive, and selective for detection and semiquantitative determination of even 5-10% kerosene in petrol.

J. Planar Chromatogr. 10, 336-341 (1997). Description of a TLC technique for quantitative determination of hydrocarbons in waste water after extraction with n-heptane by means of a micro separator. TLC on silica with n-heptane and chamber saturation. Quantification by IR spectroscopy or after dyeing with acid violet 17 which gives detection sensitivities lower by at least one magnitude. The proposed technique also enables determination of other groups of potential contaminants by applying multiple step development.

Biochemical Systematics and Ecology 26, 117-123 (1998). TLC and OPLC of formaldemethone on silica gel with chloroform. Visualization under UV 254 nm.

Anal. Chem. 72, 1759-1766 (2000). TLC of n-alkanes on silica gel and on berberine sulfate and 2,4,5,7-tetranitro-9-fluorenone impregnated silica gel (impregnation with a methanolic solution of 2 - 6 mg/100 ml of the corresponding compound for 20 s). Developed with n-hexane. Detection by densitometry at 365 nm. - An ion - molecule interaction between alkanes and berberine sulfate is considered as being responsible for the enhancement of fluorescence produced by alkanes.

J. Planar Chromatogr. 14, 343-346 (2001). HPTLC of C 60 and C 70 fullerenes on silica gel and silica gel plates impregnated with aqueous solutions of glucose, fructose, sucrose, Dextran and DVB. The mobile phase used was hexane after development with toluene to a distance of 0.5 cm to narrow the starting zone. Detection under UV 254 and 366 nm.