J Chromatogr A, 1619, 460945 (2020). Samples were lamotrigin as standard, or extracted with an oil-in-water microemulsion (10 µL butyl acetate, 4 mL n-butanol, 925 mg sodium dodecyl sulphate, 8.6 mL water) either from patients’ raw plasma (for separation from blood proteins) after spiking, or from commercial tablets dissolved in methanol. TLC on silica gel with a water-in-oil microemulsion of 9 mL butyl acetate, 1 mL n-butanol, 250 mg sodium dodecyl sulphate, 250 µL water. Both optimal microemulsions were predicted using Taguchi orthogonal array and Plackett-Burman design. Evaluation in UV 254 nm, quantification from the digital picture using four image processing software programs. For lamotrigin (hRF 24), limits of quantification were 170 ng for pure drug and 10 ng for spiked plasma. Linearity (in range 20–200 ng/spot) was directly obtained for the calibration curve in spiked plasma; however, for pure drug, linearity was obtained only when using log values of the calculated densities (300–3000 ng/spot).

J Chromatogr A, 1640, 461929 (2021). Study of the impact of different strains, culture media and parameters (temperature, time, rotational speed, and glucide and amino-acid supply) on the metabolite profile of bacteria. Samples were cultivation broths of Bacillus subtilis, B. licheniformis, B. pumilus and B. amyloliquefaciens, as well as their respective supernatant liquid-liquid extracts (apolar solvents only or QuEChERS method with acetonitrile and MgSO4 – NaCl mixture 4:1). HPTLC on silica gel (normal phase and RP-18), either as bands (for small volumes of extracts) or as areas for supernatants and bigger volumes of extracts. Extract areas were focused with a three-step procedure (up to 20mm with acetone, and twice with methanol); unextracted supernatants were focused twice with methanol and once with tetrahydrofuran, but the application zone of the plate had to be cut before development, due to the high matrix load. Development with ethyl acetate – methanol – water at different ratios after activation of the plate surface with magnesium chloride (33% relative humidity), evaluation in white light and UV. Detection of antibacterial compounds with Aliivibrio fischeri bioassay. Derivatization with primuline (for lipophilic substances) and diphenylamine aniline sulfuric acid reagent (for saccharides). This method allowed a fast comparison: A) of the patterns of the different strains (presence /absence and intensity of detected or antibacterial bands); B) of cultivation parameters: the number of metabolites increased with time, rotational speed (oxygen level), and at 37°C (vs. 30°C), whereas a minimal medium allowed the detection of more metabolites, due to the lower matrix load; C) of the impact of the extraction parameters: choice of the solvents (QuEChERS method had no advantage here), solvent – supernatant ratio (1:3 showed richer patterns than 1:1); D) of the HPTLC parameters used (better separation and resolution with normal phase vs. RP18 layers).

J. Liq. Chromatogr. Relat. Technol. https://doi.org/10.1080/10826076.2021.1933025 (2021). Laser fabrication of open capillary microchannels on cyclic olefin polymer susbtrates as stationary phase. Microchannels of 24 µm X 68 µm depth were produced using a neodymium-doped yttrium aluminum garnetan laser (Nd:YAG). The ultra thin layer chromatography layer was used for the separation of Fast Green for food coloring (1) and rhodamine 6G (2) and compared with a comercial RP-TLC. The hRF values for (1) and (2) were 9 and 18 using the UTLC plate and 10 and 57 with the RP-TLC plate. The paper highlighted the potential to tailor the surface chemistry through known surface functionalization routes for cyclic olefin polymer susbtrates that will allow unique applications for the UTLC plate.

Chromatographia 20, 223-227 (1985). Study of the overloaded systems of heptane + methylene chloride - silica using an equilibrium sandwich chamber for continuous TLC and dye mixtures as the model samples. Investigation of the effect of sample concentration and volume on the maximum separation yield. Illustration of band compression effects for samples dissolved in solvents having a low eluent strength.

J. Sliwiok (ed.): Acta Chromatographica 10, 85-96 (2000). Investigation of chemically bonded stationary phases using Raman spectroscopy to determine the density of coverage of the inorganic silica matrix with organic ligands. Comparison of Raman spectra obtained by irradiation with a high-power neodymium laser and a low-power argon-krypton ion laser.

An interesting reagent for flavonoid determination.) Use of a methanolic solution of 1% aminoethanol diphenylborate and 5 % PEG 400 for detection of various flavonoids following TLC on silica. Fluorescent spots under 366 nm. Detection limit at ng range. Discussion of the correlation between the colour of the fluorescence, Rf values and structure.

J. Chromatogr. B, 757 (2), 317-324 (2001). Description of a non-radioactive micro-assay for the cyclic phosphodiesterase reaction catalyzed by Bacillus cereus phosphatidylinositol-specific phospholipase C, involving HPTLC on silica gel to resolve the substrate (myo-inositol 1,2-cyclic phosphate and the product (myo-inositol 1-phosphate), followed by detection with a lead tetraacetate-fluorescein stain. Quantitation by analysis of the fluorescent plate image with a commercial phosphoimager and associated software, for sample in the rage of 0.1 to 50 nmol.

J. High Resol. Chromatogr. 9, 218-223 (1986). Description of a preparative anticircular chromatography (PACH) apparatus including sampling device; evaluation of sample collection techniques, quantification and evaluation of consistency by UV/VIS scanning after spot-visualization as well as application examples.