J. Planar Chromatogr. 19, 252-254 (2006). TLC of 22 amino acids on silica gel with n-propanol - water 7:3. Detection by spraying with 0.25 % 2,3-dichloro-1,4-naphthoquinone in ethanol, followed by drying in the air at room temperature and heating in an oven at 110 °C for 10 min. After cooling spraying with 0.4 % isatin in ethanol. Visual detection of spot colors (varying from yellow, to orange, pink, purple, and gray). Detection limits were determined visually and ranged from 0.01 µg (cystine and arginine) to 0.30 µg (isoleucine, phenylalanine, methionine, aspartic acid, and glycine).

J. Planar Chromatogr. 26, 202-208 (2013). HPTLC of egg yolk lipid fractions on silica gel with hexane - diethyl ether - formic acid 40:10:1. Detection by srpaying with 10 % copper(II) sulfate in 8 % phosphoric acid. Quantitation by scanning with a flatbed scanner and a gel analysis software. Intermediate/interday/intra-day precision was below 10 % CV (n=6).

Anal. Chem 55, 1858-1862 (1983). Description of a single beam imaging spectrophotometer capable of recording the reflectance or transmittance spectra of up to 64000 positions on an object within a few minutes. The transmission image is recorded by a SIT vidicon interface to a digital computer. Data can be presented as a three-dimensional array and stored on disc for later postrun processing; quantitatively and qualitatively. Preliminary results show that the method should be suitable for quantifying submicrogram fractions on TLC plates, even when chromatographic resolution is incomplete.

H. TRAITLER, A. STUDER, R.E. KAISER (eds): Instrumental HPTLC, Institute for Chromatography, Bad Dürkheim, FRG (1987), 321-329. Description of the hardware and software for quantitative evaluation of TLC with image processing scanner taking the quantitative evaluation of (-)-a-bisabolol in essential oil as example. Detection limit 200 ng. Standard deviation of measurements 3%.

J. Chromatogr. 463, 139-145 (1989). Description of a new quantification method, laser pyrolysis, for TLC without the need of visualization process in detecting any organic compound. Use of 2 amino acids, 2 pesticides as test samples. Complete analysis time less than 20 min. Comparison of sensitivity and linearity with densitometry. This technique combines the advantage of the separation power of TLC and GC detection methods. Detection limits, 100 ng for phenylalanine with FID and 20 ng for methoxychlor with ECD.

J. Planar Chromatogr. 2, 198-202 (1989). A new detector (Digital Autoradiograph, DAR) has been developed which measures, quantitatively position and intensity of 2-dimensional distributions of ionizing radiation from radioisotope labelled TLC plates. Examples and results of typical applications are presented, e.g. metabolites of pesticides or chloramphenicol-acetyl-transferase assay.

J. Planar Chromatogr. 6, 487-491 (1993). Study on the possibilities of using liquid crystals to perform quantitative determinations of organic compounds separated by TLC. Quantitative analysis reduces to simple measurements of the temperature at which the spot of the substance to be determined appears as mapped on a liquid crystalline detector.

Proc. 9th Internat. Symp. Instr. Chromatogr., Interlaken, April 9.-11., 147-154 (1997). A summary of the most important TLC-DAR (digital autoradiography technique) and NON TLC-DAR applications including different types of biological sample analysis in metabolism, pharmacokinetic, pharmacological and biochemical studies as well as in the chemical analysis of labeled compounds is given. One of the most important practical application of TLC-DAR technique is the utilization in preliminary in vitro and quantitative and comprehensive in vivo metabolism studies. Example is a metabolism study of deramciclane with TLC-DAR (TLC of extracted rats urine samples on silica with butanol - acetic acid - water 4:1:1). Measuring conditions are given in detail.