Anal. Chem. 68, 3885-3891 (1996). Investigation of the use of a scientifically operated charge-coupled device (CCD) for the detection and quantification of aflatoxins on a HPTLC plate. Use of a nebulizer-based sample application system to transfer the sample quantitatively onto the plate. Accomplishment of fluorescence excitation of the aflatoxins with a transilluminator, which caused the analytes to emit in the blue-green portion of the visible spectrum. Evaluation of the dynamic range, sensitivity, accuracy and precision of the system. Detection limits in the low picogram range.

J. Planar Chromatogr. 14, 109-112 (2001). A computer-driven office scanner has been modified to enable measurement of the fluorescence of aflatoxins on TLC plates. The main modifications were substitution of the light tube with a black light tube and inclusion of a filter. The modified scanner can be used to determine aflatoxins at low nanogram levels which, when used in combination with the appropriate TLC method, enables monitoring of the compounds in food and feed at the levels stipulated in European legislation.

CBS 96, 11-13 (2006). HPTLC of isopropylthioxanthone (ITX) in food, on silica gel in horizontal developing chamber with toluene - n-hexane 4:1 over 50 mm. Quantitative determination by fluorescence measurement at UV 254/>400 nm. Polynomial calibration via peak height, working range was 20 - 200 µg/kg. LOD is 64 pg (n=3) and in spiked fatty matrix 1 µg/kg. Positive findings were confirmed by ESI-MS in selective ion monitoring mode at m/z 255 and 277 using a plunger-based extraction device. Further confirmation by DART directly coupled to TOF-MS.

Proc. 6th Int. Symp. Instrum. Planar Chromatogr., (Interlaken 1991), Inst. Chromatogr., Bad Dürkheim, FRG, 133-135 (1991). A standard procedure is presented to take photos from TLC chromatograms in visible as well as in UV-light of 245 and 366 nm. Technical data (camera, lens, filter, film) are given.

J. Planar Chromatogr. 10, 263-266 (1997). Description of a novel, automated device for collecting fractions from TLC plates. The adsorbent is removed by scraping and then transferred by suction into empty solid-phase extraction (SPE) cartridges. The position of tracks and bands on the plate are defined by importing data from a scanning densitometer. The operator can select the bands to be collected in each SPE tube. After automatic processing, solvent extraction can be used to retrieve the analytes from the silica in the cartridges.

J. Chromatogr. A 1155 (1), 112-123 (2007). Presentation of a novel strategy for the fractionation of complex peptide mixtures using two-dimensional planar electrochromatography/thin-layer chromatography (2D PEC/TLC). It was found that phosphopeptides migrate more slowly in the first dimension, based upon their anionic phosphate residues, and certain predominantly acidic phosphopeptides even migrate in the opposite direction, relative to the bulk of the peptides. Further distinguishing phosphopeptides based upon hydrophilicity in the second dimension, which permits a restricted region of the plate to be directly interrogated for the presence of phosphopeptides by MS. Discussion of peptide sequencing and identification of phosphopeptide from the plates by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF)-MS and tandem MS.

Budapest Chromatography Conference, June 2, 1983. Review article. Application of overpressured TLC, comparison of TLC and HPTLC, a novel type of overpressured thin-layer chromatography.

(Introduction to quantitative thin-layer chromatography: basics, possibilities, automation (part 8). Kontakte 2, 40-45 (1983). This paper deals with a summary of the different types of errors in quantitative TLC. Errors caused by the chromatographic process, by the sorbent layer or by the evaluation techniques are discussed. While apparatus-dependent errors are generally difficult to eliminate, many errors in the evaluation techniques may be avoided. The problem of error propagation is also discussed with experimental examples (8 refs.).