CBS 110, 10-11 (2013). New HPTLC silica gel plates specially suited for coupling with MS and TLC plates for coupling with NMR. These MS-grade HPTLC plates are low in impurities and show less background noise, the layer thickness is 100 µm. HPTLC of human insulin and its secondary components on MS-grade silica gel with 2-butanol - pyridine - ammonia 25 % - water 39:34:10:26, detection under UV 366 nm after treatment with fluorescamine and by elution with the TLC-MS Interface and Q-TOF MS analysis in ESI positive mode, the eluent was acetonitrile - water 19:1. Separation and identification of insulin and desamido insulin was achieved, even though there is only 1 Da mass difference.

J. Sep. Sci. 41, 4387-4393 (2018). A new separation layer for TLC plates was developed for the separation in thin layers and direct conjugation to matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) without additional sample preparation. Best results were obtained with layers containing 19 to 29 % glycidyl methacrylate in the initial polymerization mixture.

(Thin-layer chromatographic enantiomeric resolution via ligand exchange.) GIT-Suppl. 3, 6-12 (1986). Separation of enantiomeric amino acids and dipeptides on reversed phase silica gel covered with a chiral selector (proline derivative). Typical developing solvents: methanol - water - acetonitrile 5:5:20 (running time 30 min) and 5:5:3 (running time 90 min).

J. Chromatogr. 365, 289-296 (1986). TLC of methylated lysine and arginine derivatives on various silicas with various aqueous salt solutions. Investigation of the influence of the origin of silica, impregnation and salt quality on chromatographic performance. The origin of the silica has a dominant influence on chromatographic performance, and the effect of impregnation and salt quality are of secondary importance.

J. Planar Chromatogr. 2, 158-160 (1989). TLC of lypophilic fraction of hydrobionts on different commercial plates with various physical characteristics. For hydrocarbons (hexadecylbenzene, dimethyl naphthalene, phenanthrene and 3,4 a-benzopyrene) the selectivity does not change with the grain diameter of the sorbent (5 - 25 µm). The Rf-value is markedly influenced by chamber saturation and specific surface areas. Plates containing a silicium acid sol as binder may be used 10 - 12 times after regeneration (increase of reproducibility).

Chromatogr. Sci. 52, 5-137 (1990). A review with 80 references on TLC stationary phase, such as silica gel, alumina, Kieselguhr, cellulose, polyamide, chemically bonded gels, etc.

J. Planar Chromatogr. 3, 169-172 (1990). Investigation of the chromatographic behavior of a wide range of compounds (ecdysteroids, dihydroxybenzoic acids, aromatic amines and N-oxides, paracetamol and metabolites) on silica and diol-bonded silica with solvent systems based on chloroform containing varying proportions of ethanol to adjust the eluotropic strength. Clear and useful differences in selectivity between the two layers were observed. The diol phase gave greatly superior results for the chromatography of the dihydroxybenzoic acids as compared to silica gel.

Consideration of separation mechanisms of some aliphatic and aromatic amines. J. Chromatogr. 609, 419-422 (1992). Investigation of the chromatographic behavior of ten aliphatic and aromatic amines on polyacrylonitrile layer with seven aqueous solvent systems. Discussion of the separation mechanisms.