Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
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      130 023
      Quality standard of traditional Chinese medicines: comparison between European Pharmacopoeia and Chinese Pharmacopoeia and recent advances
      F. LEONG (Leong Fong), X. HUA (Hua Xue), M. WANG (Wang Mei), T. CHEN (Chen Tongkai), Y. SONG (Song Yuelin), P. TU (Tu Pengfei), X. CHEN (Chen Xiao-Jia)* (*State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, Macao, China; xiaojiachen@um.edu.mo)

      Chinese Medicine 15, 76 (2020). This review compared the 2020 editions of Chinese (ChP) and European Pharmacopoeas (EuP) in different aspects of quality control of traditional Chinese medicinal plants (73 of which drugs were common to both, but with differences in species or organs for 17 of them). Discussed points included history, identification, plant origin and processing, sample preparation, marker selection, tests and assays, as well as advanced analytical techniques for quality control and for the establishment of comprehensive quality standard. TLC was discussed in relation to its following aspects: purposes, markers/references, techniques and result description.
      (A) The main uses of TLC and HPTLC were (1) chemical-based identification of the plant in a more accurate and precise method than by macroscopic and microscopic observation only, and in a more direct and easily interpretation than HPLC, and allowing the simultaneous analysis of multiple samples in parallel; (2) control of possible adulterants; (3) quantification of active compounds. Both uses (1) and (2) were combined in some EuP monographs: as example were given the roots of Angelica dahurica, A. pubescens, A. sinensis, using TLC for identification of the species and of adulterants from other species (Angelica, Levisticum and Ligusticum).
      (B) In ChP, identification through TLC was in most cases achieved by fingerprint comparison to an official reference extract or herb (herbal reference substance). At the opposite, EuP often indicated analytical markers, irrespective of any pharmacological activity, but chosen only for analytical purposes in TCM identification and quantification. Examples were: aescin and arbutin as analytical markers for TLC identification of Anemarrhena asphodeloides rhizome and Panax notoginseng root.
      For the TLC system suitability assessment tests, ChP used the same intensity markers or active markers that were chosen for the identification or assay; whereas EuP often used other specific references, e.g. isoeugenol and methyleugenol in the case of Ophiopogon japonicus roots.
      (C) For the techniques, conventional separations and chemical derivatizations were used. Hyphenations of TLC to other analytical methods (e.g. MS) were absent. Only one monograph applied an effect-directed analysis directly on TLC chromatogram (free DPPH• radical scavenging assay for TLC identification of Rehmannia glutinosa root, in ChP).
      Sometimes, the TLC methods were different between both reference books for the same species. Example was given for Belamcanda chinensis (=Iris domestica) rhizome: in EuP, development on silica gel with cyclohexane – ethyl acetate – acetic acid 20:80:1, detection under UV 254 nm, comparison to standards coumarin and irisflorentin; whereas in ChP, development on polyamide layer with chloroform – butanone – methanol (3:1:1), detection under UV 365nm after derivatization with aluminium chloride, comparison to a reference rhizome powder.
       (D) Finally, the results in ChP were described as a text stating the similarity of sample profile with the profile of the chosen reference, whereas the results in EuP were described with a schematic box indicating the positions of bands of interest.

      Classification: 1, 2a, 32e
      130 107
      Nonnegative principal component analysis in thin layer fingerprint screening: A case of Gentiana extracts from in vitro cultures
      S. GADOWSKI, K. TOMICZAK, L. KOMSTA* (*Department of Medicinal Chemistry, Faculty of Pharmacy, Medical University of Lublin, Lublin, ukasz.komsta@umlub.pl)

      J. Liq. Chromatogr. Relat. Technol. 44, 820-828 (2021). HPTLC of Gentiana extracts from in vitro cultures on silica gel with acetate - methanol - water 4:1:1 in sandwich mode. Detection under UV light at 254 nm and fluorescence at 312 nm (emission above 370 nm). Principal component analysis (PCA), hierarchical cluster analysis and the novel proposal—nonnegative PCA was performed to identify common and distinct features.

      Classification: 2d
      130 135
      Applications of the solvation parameter model in thin‑layer chromatography
      C. POOLE (Department of Chemistry, Wayne State University, Detroit,
      MI 48202, USA, cfp@chem.wayne.edu)

      J. Planar Chromatogr. 35, 207-227 (2022). The paper discussed the applications of the solvation parameter model in TLC, as a transfer model for biphasic systems in which isotropic interactions in each phase are responsible for the preferential solvation of a solute in one phase over the other. The model emphasizes the importance of water in the mobile phase and the general contributions of mobile phase interactions on the retention mechanism. In addition, the selected solvation of the stationary phase in contact with the mobile phase makes an important contribution to system selectivity. System constants for reversed-phase separations on chemically bonded silica gel HPTLC layers with different mobile phases compositions were also discussed and applied to construct correlation diagrams to facilitate the comparison of selectivity for different layers with the same mobile phase composition or different mobile phase compositions for the same layer.

      Keywords: HPTLC
      Classification: 2c
      130 019
      Micellar thin layer chromatography and computer-aided analysis of empagliflozin, linagliptin and metformin HCl ternary mixture
      N.S. ABBAS, Y. A. S. MOHAMED*, S.M. DERAYEA, M.A. OMAR, G.A. SALEH (*Dep. of Med. & Pharm. Anal. Chem., Fac. of Pharmacy - Sana'a Univ., Sana'a, Yemen, gadeed2017@yahoo.com)

      J Chromatogr Sci, 60 (10), 946 - 952 (2022). Study of the mechanism of micellar thin layer chromatography (MTLC) and development of a new simple and sensitive method for simultaneous separation of empagliflozin, linagliptin and metformin hydrochloride ternary mixture. TLC using a micellar mobile phase composed of methanol and aqueous solution of each of three different surfactants, sodium dodecyl sulphate (SDS), benzalkonium chloride (BAC) and polysorbate 80 (tween 80), in ratio of 2:3. Micellar TLC determination at 237 nm. Investigation of the quantitative structure - retention relationships by modeling, evaluating and validating with a molecular operating environment software. Discussion of the basis for selecting surfactants, for example the separation using SDS (anionic surfactant) and BAC (cationic surfactant) depended on ionization potential (AMI-IP), partition coefficient (logP (o/w)) and hydrogen bond donor atoms (a-don), whereas the separation using tween 80 depended mainly on the lipophilicity (RM0), solvation energy (E-sol) and Van der Waals energy (E-vdw).

      Classification: 2, 32a
      130 015
      Mixed-mode hydrophilic interactions/reversed-phase retention mechanism in thin-layer chromatography
      Darija OBRADOVIC*, T. KOWALSKA, D. AGBABA (*Dep. of Pharm. Chem., Univ. of Belgrade-Fac. of Pharmacy, Belgrade, Serbia, darija2207@gmail.com; darija@pharmacy.bg.ac.rs)

      J Chromatogr Sci, 60 (4), 372-386 (2022). Investigation of the dual retention mechanism in TLC taking place on three stationary phases of different polarity (C-18, silica gel and DIOL)  using binary mobile phases composed of acetonitrile as the main component and water, or methanol as a modifier. The test analytes were 12 compounds of pharmaceutical importance and considerably different chemical structure, i.e. the imidazoline and serotonin receptor ligands, and their related compounds. Determination of retention of each analyte in each investigated chromatographic system in a wide enough range of the mobile phase composition, with volume fraction of the mobile phase modifier ranging from 0.10 to 0.90. Calculation of the exact turning point values as a proof of occurrence of the reversed-phase hydrophilic interaction chromatography (HILIC/RP) retention mechanism based on the multimodal retention model. Analysis of the dual retention mode with the use of the volume fraction of the mobile phase modifier, the total polarity and the total solubility models, allowing the dual (HILIC/RP) retention mechanism for the DIOL, C-18 and silica gel stationary phase to be confirmed. The observed retention mechanism was more complicated than the dual HILIC/RP one in the case of the DIOL stationary phase and acetonitrile/methanol mobile phase.

      Classification: 2d
      130 100
      Hydrophilic retention mechanism of imidazoline and serotonin receptor ligands in thin‑layer and high‑performance liquid chromatography systems
      D. OBRADOVIC*, J. SAVIC, J. JOKSIMOVIC, T. KOWALSKA, D. AGBABA (*Department of Pharmaceutical Chemistry, University, of Belgrade, Faculty of Pharmacy, Belgrade, Serbia, darija@pharmacy.bg.ac.rs)

      J. Planar Chromatogr. 35, 251-263 (2022). HPTLC of ten imidazoline and serotonin
      receptor ligands on amino phase with acetonitrile modified with different volume fractions of methanol and water as mobile phases, acidified with 20 mM ammonium acetate and 0.1 % acetic acid. Detection under UV light at 254 nm. Correlation between retention descriptors and molecular properties was analyzed and different elution orders and separation performances were obtained for the compounds, depending on the chromatographic system employed. 
       

      Classification: 2c
      130 022
      Predicting pharmacokinetic properties of potential anti‑cancer agents using micellar thin‑layer chromatography
      M. JANICKA*, A. SLIWINSKA, M. SZTANKE, K. SZTANKE (*Department of Physical Chemistry, Faculty of Chemistry, Institute of Chemical Science, Maria Curie-Skłodowska University, Lublin, Poland, malgorzata.janicka@mail.umcs.pl)

      J. Planar Chromatogr. 35, 265-272 (2022). HPTLC of newly synthesized azaisocytosine-containing congeners on RP-CN with buffered (pH = 7.4) 0.1 M sodium dodecyl sulphate with 20 % of tetrahydrofuran. Detection under UV light at 254 nm. The micellar chromatographic parameters measured using the TLC technique were proposed as lipophilicity descriptors, and, together with the molecular weight and polarizability, the descriptors were applied in the QSARs methodology to predict the compounds’ blood‒brain distribution. 

      Classification: 2c
      129 020
      Forecasting structure of natural products through color formation process by thin layer chromatography
      L. XU (Xu Lujing), S. LIU (Liu Songbai)* (*Department of Food Science and Nutrition, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology, China, songbailiu@zju.edu.cn)

      Food Chem. 334, 127496 (2021). TLC of 36 compounds of 11 classes of bioactives including polyphenols, flavonoids, phytosterols, saponin and saccharides on silica gel. The relationship between structure and color of the bioactives was studied using a broad-spectrum staining solution: p-anisaldehyde - acetic acid - 95 % ethanol - sulfuric acid 920:375:338:125, followed by heating at 250 °C for 10-20 s. Color formation was recorded by a mobile phone camera. Videos were disassembled into frames employing the functions of Matlab. 

      Classification: 2c