Application Notes

HPTLC is the method of choice for many analytical tasks - a broad range of Application Notes is available for download. Registered users can download the Application Notes for free.

CAMAG Laboratory develops and validates HPTLC methods for the identification of botanical raw materials and finished products. By following these methods reproducible results are guaranteed.

These documents are part of the appendix of the paper Validation of High-Performance Thin-Layer Chromatographic  Methods for the Identification of Botanicals in a cGMP Environment by Eike Reich, Anne Schibli, and Alison DeBatt (Journal of AOAC 91(1) 2008). This paper provides a summary of a project that aimed at:

  • development and validation of 10 HPTLC methods for identification of botanical raw materials
  • elaboration of a general concept for validation of such methods
  • proving that their validation is possible and necessary with respect to cGMP
  • providing to the botanical industry guidance in establishing practical and meaningful methods for quality assurance.

To view a validation protocol example, click here.

IDApplication Note

A-01.4 Carbamazepine and two of its metabolites in serum
A-07.3 Mono-, di-, tri- and polysaccharides
A-10.5 Vitamin C in fruit juice
A-100.1 Detection of sibutramine in slimming products by HPTLC and confirmation by HPTLC-MS
A-101.1 Determination of SARA in bitumen by HPTLC
A-102.1 Adulteration of St. Johns Wort Products: An HPTLC-MS Investigation
A-103.1 Detection of UV filters in cosmetic products by HPTLC and confirmation by HPTLC-MS
A-104.1 HPTLC fingerprint of Edelweiss plants and extracts used as ingredients in cosmeceuticals
A-105.1 Detection of steroids and SARMs
A-106.1 Quantification of nicotine in liquids for electronic cigarettes
A-107.1 Screening for phenethylamines in pre-workout supplements (boosters)
A-108.1 Identification and quantification of different cannabinoids in Cannabis sativa
A-109.1 HPTLC test for minimum content on 18β-glycyrrhizic acid (CAS 1405-86-3) in Licorice root (Glycyrrhiza glabra and Glycyrrhiza uralensis) based on the acceptance criteria of USP 39 NF 34
A-11.4 Amino acids in potatoes
A-110.1 Identification of Olive leaf (Olea europaea L.)
A-111.1 Identification of Decaffeinated Green Tea Dry Extract according to USP DSC 2018
A-112.1 Limit test for caffeine content (< 0.1% according to the USP 40 NF35)
A-113.1 Densitometric quantification of oleuropein in Olive leaf dry extracts (Olea europaea L.)
A-114.1 Identification of wintergreen oil by HPTLC and discrimination of pure (synthetic) methyl salicylate
A-115.1 Identification of Curcuminoids extract according to USP DSC 2018
A-116.1 Identification of Yerba mate (Ilex paraguariensis A.St.-Hil.)
A-117.1 Densitometric quantification of two steviol glycosides in Stevia leaf dry extract (Stevia rebaudiana (Bertoni) Bertoni)
A-118.1 Screening method for identification of forensic drugs
A-119.1 Identification of Cola nitida seed (Cola nitida (Vent.) Schott & Endl.)
A-12.4 Aflatoxins B1, B2, G1 and G2 in foodstuffs
A-120.1 Strategy for rapid separation, detection and quantification of explosives by HPTLC
A-121.1 Identification of Astragalus root (Astragalus membranaceus (Fisch.) Bunge and Astragalus membranaceus var. mongholicus (Bunge) P. K. Hsiao)
A-122.1 Identification of Garcinia gummi-gutta fruits (Garcinia gummi-gutta (L.) N. Robson)
A-123.1 Identification of Garcinia indica fruits (Garcinia indica (Thouars) Choisy)
A-124.1 Identification of Banaba leaves (Lagerstroemia speciosa (L.) Pers.)
A-125.1 Identification of Lonicera japonica flower (Lonicera japonica (Thunb.)
A-14.4 Acetylsalicylic acid, salicylic acid, and salicyl amide by HPTLC
A-15.5 p-Hydroxybenzoic acid, its esters and 4-methoxycinnamic acid derivatives
A-20.3 Trimethoprim (TPM) and sulfamethoxazole (SMZ) from cotrimoxazole (antibiotic)
A-22.2 Estriol in serum during pregnancy
A-23.2 Lecithin and sphingomyelin in amniotic fluid (L/S ratio)
A-24.2 Cortisol in serum
A-25.2 Lactose, saccharose and fructose/glucose
A-26.2 Selenium after derivatization with 2,3-diaminonaphthalene in water and serum
A-27.4 Vitamin B1 in pharmaceutical products
A-28.7 Pesticides in drinking water
A-29.3 Sulfonamides and tetracyclines in feed (AMD)
A-39.2 Hippuric acid in urine
A-41.3 Glycerol in tobacco
A-42.3 Anticonvulsants in serum
A-43.2 Hydrochlorothiazide (Esidrex®) in tablets
A-44.2 Caffeine in urine
A-46.3 Furosemide (Lasix®) in urine
A-47.3 Chlortalidone (Hygroton®) in urine
A-48.3 Atenolol [Tenormin®] in urine
A-49.3 Pemoline [Stimul®] in urine
A-50.3 Oxprenolol in blood
A-52.3 Phospholipids (AMD)
A-57.1 Morphine and monoacetylmorphine in human hair
A-60.3 Drug screening by instrumental qualitative thin-layer chromatography
A-62.1 Ball pen inks (AMD)
A-63.1 Quinolonic antibiotics in fish and fish feed
A-64.5 Carcinogenic amines as degradation products of textile azo dyes (AMD)
A-65.2 Content uniformity test with reference to diclofenac-Na (Voltaren)
A-66.1 Diflubenzuron in needles and leaves
A-70.1 Caffeine in Guarana Cofertan extract
A-72.1 Validated HPTLC method for the determination of ornidazole in plasma
A-73.1 Actein in Cimicifuga racemosa rhizoma
A-74.2 Hydrocortisone and conchocaine in lanolin ointment
A-75.1 6 PAHs in contaminated mineral substances
A-76.1 Rape seed in fermentation broth
A-77.2 Impurities of K acid
A-78.2 Organic acids in wine
A-79.1 Allergenic disperse dyes in textiles (AMD)
A-80.1 Carcinogenic amines as degradation products in leather
A-81.2 Oligosaccharides in molasses
A-82.1 Impurities in bupropion hydrochloride
A-83.1 Determination of sucralose and fructose in food and beverages by HPTLC
A-84.1 Determination of tetrandrine in Han fangji (Stephania tetrandra)
A-85.1 Determination of Capsaicin in Capsicum tincture by HPTLC
A-86.1 Artemisinin in Artemisia annua leaf
A-87.1 Determination of aucubin and catalpol in leaves of Ribwort Plantain (Plantago lanceolata)
A-88.1 Determination of melamine in milk by HPTLC
A-89.1 HPTLC method for the determination of apolar lipids from human skin
A-90.1 HPTLC method for determination of ceramides from human skin
A-91.1 Determination of phospholipids by HPTLC
A-92.1 Determination of ginkgolides A, B, C and bilobalide in Ginkgo biloba dry extract by HPTLC
A-93.1 Determination of the flavonoid rutin in Ginkgo biloba dry extract by HPTLC
A-94.1 Determination of illegal dyes in spices by HPTLC
A-95.1 Determination of metronidazole in pharmaceutical dosage form by HPTLC
A-96.1 Rapid content uniformity test of 6 batches of Coenzyme Q10 in soft gel capsules by HPTLC
A-97.1 Determination of aflatoxins B1, G1, B2 and G2 in tomato extract by HPTLC
A-98.1 Confirming the presence of cannabinoids in Cannabis sativa by HPTLC-MS
A-99.1 In-process control of ergoline psychedelics during chemical synthesis by HPTLC
F-01 Valerian (Valeriana officinalis)
F-02 Wuweizi (Schisandra chinensis)
F-03 Hawthorn (Crataegus sp.)
F-04 Astragalus and Hedysarum
F-05 Cramp Bark and Black Haw (Viburnum sp.)
F-06A Garlic (Allium sativum): Alliin
F-06B Garlic (Allium sativum): Allicin
F-07 Willow bark (Salix sp.)
F-08 Ashwaganda (Withania somnifera)
F-09 Reishi - mushrooms (Ganoderma lucidum)
F-10A Chaste tree fruits (Vitex agnus-castus): Hydrophilic flavonoids
F-10B Chaste tree fruits (Vitex agnus-castus): Lipophilic plant ingredients
F-10C Chaste tree fruits (Vitex agnus-castus): Iridoids
F-11 Bearberry leaf (Arctostaphylos uva-ursi)
F-12 Common Horse tail (Equisetum arvense)
F-13A Black Cohosh (Cimicifuga racemosa): Triterpene glycosides
F-13B Black Cohosh (Cimicifuga racemosa): Plant acids
F-14 St. John's Wort (Hypericum perforatum)
F-15 Goldenseal (Hydrastis canadensis)
F-16A Ginkgo (Ginkgo biloba): Ginkgolides
F-16B Ginkgo (Ginkgo biloba): Flavonoids
F-16C Ginkgo (Ginkgo biloba): Ginkgolic acid
F-17 Bilberry (Vaccinum myrtillus)
F-18 Dang Gui (Angelica sinensis)
F-19 Feverfew (Tanacetum parthenium)
F-20 African Prune Bark (Pygeum africanum)
F-21 Han fangji (Stephania tetrandra)
F-22A Red Clover (Trifolium pratense): Flavonoids
F-22B Red Clover (Trifolium pratense): Formononetin
F-23 HPTLC Fingerprints of Chinese plants with respect to aristolochic acids
F-24A Echinacea (E. purpurea, E. angustifolia, E. pallida): Phenolics
F-24B Echinacea (E. purpurea, E. angustifolia, E. pallida): Alkylamides
F-25 Kava root (Piper methysticum)
F-26A Saw Palmetto (Serenoa repens): Lipophilic plant ingredients
F-26B Saw Palmetto (Serenoa repens): Fatty acids
F-27 Ribwort Plantain (Plantago lanceolata )
F-28 Lime flowers, Linden (Tilias sp.)
F-29A Licorice root (Glycyrrhiza sp.): Polar compounds
F-29B Licorice root (Glycyrrhiza sp.): Lipophilic compounds
F-30 Eleuthero (Eleutherococcus senticosus)
F-31 Asian Ginseng (Panax ginseng)
F-32 American Ginseng (Panax quinquefolium)
F-33 Notoginseng (Panax notoginseng)
F-34 HPTLC-Bioluminescence screening
F-35 HPTLC detection of amaranth in Bilberry extract
F-36 HPTLC Identification of Triphala
F-37 HPTLC Identification of Fatty Oils
F-38 DPPH HPTLC Screening
F-39 HPTLC Identification of Fatty Oils USP
MOA 001 Green Tea
MOA 002 Ginseng
MOA 003 Eleuthero
MOA 004 Echinacea
MOA 005 Black Cohosh
MOA 006 Licorice
MOA 007 Kava
MOA 008 Milk Thistle
MOA 009 Feverfew
MOA 010 Ginger
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