J. Liq. Chromatogr. Relat. Technol. 46, 11-15 (2023). Review of chromatographic methods for detecting aflatoxins, including HPTLC in various matrices of food, feed, and even herbal medicines. The paper described sample preparation, quantification in herbal formulations and risk assessment.

J. Planar Chromatogr. 36, 237-249 (2023). HPTLC of the leaves of Ficus benghalensis on silica gel with toluene - ethyl acetate 93:7. Detection under UV light at 254 and 366 nm. Bioautography by pouring Bacillus subtilis culture over the plate, followed by incubation at 37 °C for 24 h. After incubation, 1 mL of 0.2 mg/mL p-iodonitrotetrazolium chloride was added to the bioautography plates and monitored at room temperature until a pink color developed. Zones of inhibition were selected and further analyzed by high-resolution liquid chromatography (HR-LC) and mass spectrometry (MS).

J. Liq. Chromatogr. Relat. Technol. 46, 1-5 (2023). HPTLC of erythromycin (1), sulfadiazine (2) and trimethoprim (3) in different edible chicken tissues on silica gel with chloroform – methanol - 33 % ammonia hydroxide solution 85:15:1. Quantitative determination by absorbance measurement at 220 nm. The hRF values for (1) to (3) were 7, 42 and 68, respectively. Linearity was in the range of 0.5-10 µg/zone for (1), 0.1-2 µg/zone for (2) and 0.1-1.8 µg/zone for (3). Intermediate precisions were below 2 % (n=3). LOD and LOQ were 200 and 500 ng/zone for (1), 30 and 90 ng/zone for (2) and 20 and 80 ng/zone for (3), respectively. Mean recovery was 99.5 % for (1), 104.1 % for (2) and 98.3 % for (3).

J. Agric. Food. Chem. 70, 10886-10898 (2022). HPTLC of 81 veterinary drugs from 6 different groups (glucocorticoids, anthelmintics, antiparasitics, coccidiostats, nonsteroidal anti-inflammatory drugs, and antibiotics) in 4 different matrices (honey, pig muscle, cow milk, and chicken eggs) on silica gel with acetonitrile - ammonia - ethanol 13:4:3 up to 80 mm and then in the reverse direction with methanol - acetonitrile - ethanol - ammonia 6:2:1:1 up to 45 mm. After each step, the plates were automatically dried in a cold stream of air for 3 min. Detection under UV light at 254 nm and fluorescence light detection (FLD) at 366 nm. Zones were eluted from the plate using a fully automated auto TLC-LC-MS interface for further analysis by high-resolution tandem mass spectrometry. Most veterinary drugs except penicillins and cephalosporins were detected at the 5 μg/kg level in pig muscle, cow milk, and chicken eggs and 25 μg/kg level in honey.

Anal. Chem. 88, 10979-10986 (2016). HPTLC of three Salvia miltiorrhiza root samples on silica gel with petroleum ether - cyclohexane - ethyl acetate 25:14:10. Detection under white light, UV light at 254 and 366 nm. Lipophilic antimicrobials were analyzed in direct combination with Aliivibrio fischeri and Bacillus subtilis bioassays. Bioactive zones were further analyzed by mass spectrometry. 

J. Sep. Sci. 45, 2582-2590 (2022). HPTLC of favipiravir (1), molnupiravir (2), and ritonavir (3) on silica gel with methylene chloride - ethyl acetate - methanol - 25 % ammonia 6:3:4:1. Quantitative determination by absorbance measurement at 289 nm. The hRF values for (1) to (3) were 22, 42 and 63, respectively. Linearity was between 4 and 100 µg/mL for (1) and (2) and 3 and 100 µg/mL for (2). Inter-day and intra-day precisions were below 2 % (n=5). The LOD and LOQ were 1 and 3 µg/mL for (1) and (2), and 1 and 4 µg/mL for (2). Mean recovery was 99.6 % for (1), 99.5 % for (2) and 99.8 % for (3). 

J. Sep. Sci. 2200951 (2022). HPTLC of amoxicillin (1), metronidazole (2), and famotidine (3) on silica gel with methanol - chloroform - toluene - water - glacial acetic acid 50:20:15:5:1. Quantitative determination by absorbance measurement at 280 nm for (1) and (3) and 300 nm for (2). The hRF values for (1) to (3) were 42, 81 and 52, respectively. Linearity was between 100 and 1600 ng /zone for (1) and 100 and 900 ng/zone for (2) and (3). Inter-day and intra-day precisions were below 2 % (n=9). The LOD and LOQ were 30 and 100 ng/zone for (1), 20 and 60 ng/zone for (2) and 30 and 90 ng/zone for (2), respectively. Mean recovery was 99.8 % for (1), 100.0 % for (2) and 101.0 % for (3).

Phytochem. Anal. 33, 1177-1189 (2022). HPTLC of 35 species from four genera
(Combretum, Pteleopsis, Quisqualis, Terminalia) of Combretaceae on silica gel with ethyl acetate - formic acid - water 50:3:3. Detection by spraying with 10 % methanolic sulfuric acid and visualization under white light. Detection of flavonoids by spraying with Natural Product Reagent and examined under UV light at 366 nm. For bioautography analysis, the developed plate was sterilized under UV light for 1 h, then overlaid with tryptone soya agar containing the appropriate bacterial culture: Staphylococcus aureus (ATCC 25923), Bacillus cereus (ATCC 11778), Escherichia coli (ATCC 8739), and Salmonella typhimurium (ATCC 14028). Active compounds were identified by mass spectrometry.