Parameters of Planar Chromatography / TLC useful hints

The articles in this series are dedicated to the important steps of thin-layer chromatography and their parameters which influence the chromatographic result. Hints for optimization are given to help the reader to use thin layer chromatography most efficiently.

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• Proposal for a standardized approach to HPTLC (SOP)
• Handling of plates
• Sample Application
• Chromatogram development
  Part 1: Chamber type and chamber saturation
• Chromatogram development
  Part 2: Effects of developing distance and position in the chromatogram on resolution
• Documentation of TLC/HPTLC plates
• Derivatization
• Scanning Densitometry
  Part 1: Scanning modes and multi-wavelength scan