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Sample Application

 

Application of samples, reference standards and/or reference materials is the first step in the workflow of TLC/HPTLC and it affects significantly the quality of the result at the end of the process.

The choice of the application technique and the device depend on the requirements of precision, sample volumes, number of analyses and the desired grade of automation.

Spotwise sample application using a fixed volume capillary is the simplest way. Sample volumes of 0.5 to 5 μL can be applied as spots onto conventional layers without intermediate drying, on HPTLC layers it is up to 1 μL per spot. It is recommended to guide the capillary by means of the Nanomat 4.

Bandwise application by spray-on techniques allows the application of significantly larger volumes. Starting zones in the form of narrow bands ensure the best resolution that can be achieved with the chromatographic system selected.

Very large sample volumes or samples with a high matrix content can be sprayed-on in the form of rectangles with the Automatic TLC Sampler (ATS 4) which, prior to chromatography, are focused into narrow bands by a short development step with a solvent of high elution strength.

Effect of the solvent and the technique of sample application on the chromatogram

Products

Nanomat 4 and Capillary Dispenser
The CAMAG Nanomat 4 serves for easy application of samples in the form of spots onto TLC and HPTLC plates, precisely positioned and without damage to the layer.
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Linomat 5
With the CAMAG Linomat 5 samples are sprayed onto TLC/HPTLC plates in the form of bands with nitrogen or compressed air. Sample application is automatic, only changing the syringe (filling, inserting and rinsing) is manual. The Linomat is suitable for small sample throughput.
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Automatic TLC Sampler 4 (ATS 4)
Automatic sample application is a key factor for productivity of the HPTLC laboratory. The ATS 4 offers fully automatic sample application for qualitative and quantitative analyses as well as for preparative separations. It is suited for routine use and high sample throughput in mass analysis.
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