Application Notes

HPTLC is the method of choice for many analytical tasks - a broad range of Application Notes is available for download. Registered users can download the Application Notes for free.

CAMAG Laboratory develops and validates HPTLC methods for the identification of botanical raw materials and finished products. By following these methods reproducible results are guaranteed.

These documents are part of the appendix of the paper Validation of High-Performance Thin-Layer Chromatographic  Methods for the Identification of Botanicals in a cGMP Environment by Eike Reich, Anne Schibli, and Alison DeBatt (Journal of AOAC 91(1) 2008). This paper provides a summary of a project that aimed at:

  • development and validation of 10 HPTLC methods for identification of botanical raw materials
  • elaboration of a general concept for validation of such methods
  • proving that their validation is possible and necessary with respect to cGMP
  • providing to the botanical industry guidance in establishing practical and meaningful methods for quality assurance.

To view a validation protocol example, click here.

IDApplication Note

A-01.4 Carbamazepine and two of its metabolites in serum
A-100.1 Detection of sibutramine in slimming products by HPTLC and confirmation by HPTLC-MS
A-101.1 Determination of SARA in bitumen by HPTLC
A-103.1 Detection of UV filters in cosmetic products by HPTLC and confirmation by HPTLC-MS
A-105.1 Detection of steroids and SARMs
A-106.1 Quantification of nicotine in liquids for electronic cigarettes
A-107.1 Screening for phenethylamines in pre-workout supplements (boosters)
A-118.1 Screening method for identification of forensic drugs
A-120.1 Strategy for rapid separation, detection and quantification of explosives by HPTLC
A-14.4 Acetylsalicylic acid, salicylic acid, and salicyl amide by HPTLC
A-15.5 p-Hydroxybenzoic acid, its esters and 4-methoxycinnamic acid derivatives
A-20.3 Trimethoprim (TPM) and sulfamethoxazole (SMZ) from cotrimoxazole (antibiotic)
A-22.2 Estriol in serum during pregnancy
A-23.2 Lecithin and sphingomyelin in amniotic fluid (L/S ratio)
A-24.2 Cortisol in serum
A-25.2 Lactose, saccharose and fructose/glucose
A-26.2 Selenium after derivatization with 2,3-diaminonaphthalene in water and serum
A-27.4 Vitamin B1 in pharmaceutical products
A-28.7 Pesticides in drinking water
A-39.2 Hippuric acid in urine
A-41.3 Glycerol in tobacco
A-42.3 Anticonvulsants in serum
A-43.2 Hydrochlorothiazide (Esidrex®) in tablets
A-44.2 Caffeine in urine
A-46.3 Furosemide (Lasix®) in urine
A-47.3 Chlortalidone (Hygroton®) in urine
A-48.3 Atenolol [Tenormin®] in urine
A-49.3 Pemoline [Stimul®] in urine
A-50.3 Oxprenolol in blood
A-52.3 Phospholipids (AMD)
A-57.1 Morphine and monoacetylmorphine in human hair
A-60.3 Drug screening by instrumental qualitative thin-layer chromatography
A-62.1 Ball pen inks (AMD)
A-63.1 Quinolonic antibiotics in fish and fish feed
A-64.5 Carcinogenic amines as degradation products of textile azo dyes (AMD)
A-65.2 Content uniformity test with reference to diclofenac-Na (Voltaren)
A-66.1 Diflubenzuron in needles and leaves
A-70.1 Caffeine in Guarana Cofertan extract
A-72.1 Validated HPTLC method for the determination of ornidazole in plasma
A-73.1 Actein in Cimicifuga racemosa rhizoma
A-74.2 Hydrocortisone and conchocaine in lanolin ointment
A-75.1 6 PAHs in contaminated mineral substances
A-76.1 Rape seed in fermentation broth
A-77.2 Impurities of K acid
A-79.1 Allergenic disperse dyes in textiles (AMD)
A-80.1 Carcinogenic amines as degradation products in leather
A-82.1 Impurities in bupropion hydrochloride
A-89.1 HPTLC method for the determination of apolar lipids from human skin
A-90.1 HPTLC method for determination of ceramides from human skin
A-91.1 Determination of phospholipids by HPTLC
A-95.1 Determination of metronidazole in pharmaceutical dosage form by HPTLC
A-96.1 Rapid content uniformity test of 6 batches of Coenzyme Q10 in soft gel capsules by HPTLC
A-98.1 Confirming the presence of cannabinoids in Cannabis sativa by HPTLC-MS
A-99.1 In-process control of ergoline psychedelics during chemical synthesis by HPTLC
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