Phospholipids are essential players in the creation of bilayer membranes within living organisms. Their unique ability to form structures like liposomes or micelles, which can encapsulate active ingredients, is highly valued in the pharmaceutical and cosmetic industries. Moreover, the food sector is experiencing a growing need to accurately measure phospholipids to highlight the preventative and healing properties of these dietary components. This method excels at both detecting and quantifying a variety of phospholipids, including LPC, SM, PC, PS, PI, PA, PG, PE, and NAPE.